rabbit anti nfκb p p65  (Bioss)

Journal: Poultry Science

Article Title: CSF3 enhances the innate immune responses to ALV-J infections via NF-κB and interferon pathways

doi: 10.1016/j.psj.2025.105648

Figure Lengend Snippet: CSF3 enhances the immune response to ALV-J via the NFκB signaling pathway. (A) Western blot analysis of p52, p100, phosphorylated p65 (p-p65), IκBα, and phosphorylated IκBα (p-IκBα) protein following CSF3 overexpression in DF-1 cells. (B) Quantification of protein levels in (A) based on relative grayscale values. (C) Western blot analysis of p52, p100, phosphorylated p65 (p-p65), IκBα, and phosphorylated IκBα (p-IκBα) protein following CSF3 knockdown in DF-1 cells. (D) Quantification of protein levels in (C) based on relative grayscale values. (D, E) RT-qPCR analysis of TNF-α, IL-1β , and IL-6 mRNA expression following CSF3 overexpression (D) or knockdown (E) in DF-1 cells. (F, G) ELISA measurement of TNF-α, IL-1β, and IL-6 protein levels following CSF3 overexpression (F) or knockdown (G) in DF-1 cells. (H, I) RT-qPCR analysis of TNF-α, IL-1β , and IL-6 mRNA expression following CSF3 overexpression (H) or knockdown (I) in CEF cells. (J, K) ELISA measurement of TNF-α, IL-1β, and IL-6 protein levels following CSF3 overexpression (J) or knockdown (K) in CEF cells. (L) Western blot analysis of STAT3, phosphorylated STAT3 (p-STAT3), env, β-actin, IκBα, p-IκBα, p-p65, p52, and p100 after STAT3 phosphorylation inhibition in CSF3-overexpressing DF-1 cells. (M) Quantification of protein levels in (L) based on relative grayscale values. Statistical significance was determined using a two-tailed unpaired Student’s t-test ( p < 0.05). * p < 0.05, **p < 0.01, *** p < 0.001.

Article Snippet: Mouse Anti-ALV-J envelope protein JE9 (kindly provided by Prof. Aijian Qin, Yangzhou University, Yangzhou, China), Rabbit Anti-STAT3 antibody (bs-1141R; Boss, China; 1:1000), Rabbit Anti-phospho-STAT3 (Ser727) antibody (bs-3429R; Boss, China; 1:1000), Rabbit Anti-NFκB2 antibody (10037P, Boss, China; 1:1000), Rabbit Anti-NFκB p-p65 (bs-0982R, Boss, China; 1:1000), Rabbit Anti-IKBα Rabbit (10268-1-AP, Proteintech, USA; 1:1000), Anti-p-IKBα (bs-2513R, Boss, China; 1:1000), and goat anti-rabbit IgG/HRP (bs13278), goat anti-mouse IgG/HRP (bs12478), goat Anti-Mouse IgG ( H + L ) FITC (bs10950) secondary antibody were purchased from Bioss (Beijing, China).

Techniques: Western Blot, Over Expression, Knockdown, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Inhibition, Two Tailed Test

Journal: Antioxidants

Article Title: 6- O - trans -feruloyl Catalpol, a Natural Antioxidant from the Stem Bark of Catalpa ovata , Accelerates Liver Regeneration In Vivo via Activation of Hepatocyte Proliferation Signaling Pathways

doi: 10.3390/antiox14101210

Figure Lengend Snippet: 6- O-trans -feruloyl catalpol promotes the activation of the NF-κB signaling pathway in mice after 2/3 Partial hepatectomy. ( A ) Effect of 6FC on NF-κB-related protein levels. Representative immunoblots show the expression levels of p-IKKα, p-IκBα, and p-NF-κB p65 in liver tissues post-Partial hepatectomy. Densitometric analysis was performed using ImageJ software to quantify the relative expression levels of these proteins. ( B ) Effects of 6- O-trans -feruloyl catalpol on the mRNA levels of NF-κB downstream target genes. Real-time PCR analysis was conducted to evaluate the expression of NF-κB-regulated genes in liver tissues after Partial hepatectomy. Data are presented as the mean ± SD ( n ≥ 5). + p < 0.05 vs. vehicle-treated group. * p < 0.05 vs. silymarin-treated group.

Article Snippet: The primary antibodies used in this study included p-IKKα (#2859s), p-IKBα (#2895S), p-NFκB p65 (Ser536) (#3033_ 93H1), NFκB p65 (#8242_ D14E12), p-AKT (Ser473) (#9271), p-ERK (#9101), ERK (#9102), p-JNK (#9251), p-p38 (#9211s), p-Stat3 (#9145_D3A7) and Stat3 (#4904_79D7) were purchased from CST (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Expressing, Software, Real-time Polymerase Chain Reaction